沙苑子調節小鼠免疫功能作用的實驗研究(doc 7)

關鍵詞：沙苑

【摘要】 目的 探討沙苑子及其複方製劑對小鼠免疫功能的調節作用。方法 二級昆明小鼠174隻，雌雄各半，體重18～22g，隨機分3組。用沙苑子及其複方製劑給小鼠灌胃15d後活殺，用稱重法測量髒器係數；用足蹠厚度增加法評價細胞免疫功能；用溶血空斑法評價體液免疫功能；用腹腔巨噬細胞吞噬雞紅細胞的方法評價巨噬細胞吞噬功能。結果 沙苑子能明顯提高小鼠DTH反應程度；巨噬細胞吞噬功能明顯增強；溶血空斑數明顯增多；白細胞介素-1、6、8(IL-1、6、8)水平增高，與對照組比較具有顯著的統計學意義(P＜0.05)。結論 沙苑子具有調節小鼠免疫功能的作用。

【關鍵詞】 沙苑子；免疫功能；小鼠

The effect of flatstem milkvetch seed and its compound preparation on

regulation of immunological function of mice


【Abstract】 Objective To investigate the effect of flatstem milkvetch seed and its compound preparation on the regulation of immunological function of mice.Methods One hundred seventy four of second order Kunming mice(weight 18～22g)，male and female each occupying one half，were randomly divided into three groups.All the mice were sacrificed after they had been given flatstem milkvetch seed and its compound preparation in intragastric administration for fifteen days.The organ’s index of mice was calculated by determining the organ’s weight and the cell immunity were evaluated by determining the thickening of pedis.Hemolytic plaque test was used to appraise humoral immunity，and peritoneal macrophage swallowing chicken red blood cell test was used to appraise the phagocytes function.Results Compared with the control group，the DTH reaction and phagocytes function were improved obviously(P＜0.05)，and the quantity of hemolytic plaque and the concentration of IL-1，IL-6，IL-8 were increased significantly(P＜0.05) in flatstem milkvetch seed and its compound preparation group.The spleen and thymus index had no statistic difference among all the groups.Conclusion Flatstem milkvetch seed can regulate the immunological function of mice.
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